The right answers to frequently asked questions
Find the answers to all our products and services by clicking the links below.
General Questions regarding Sample Submission, Ordering & Sample Shipment & Results can be found here:
What kind of quality control do you perform?
NGSelect Amplicon on NovaSeq (150 - 270 bp):
Prior to sequencing, the quality and quantity of each sample will be determined by established methods (e.g. agarose gel analysis / Qubit® Fluorometer / NanoDrop / Agilent 2100 Bioanalyzer). Further quality controls are performed at various steps of the sequencing workflow.
NGSelect Amplicon on MiSeq (280 - 570 bp):
Beside the evaluation of the gel picture you are sending, the success of the PCR reaction serves as our quality control. After the library prep and at various steps of the sequencing workflow further quality controls are performed.
What should I do if my sample fails the entry QC?
NGSelect Amplicon Adaptor Ligation:
If the amount, concentration or quality of the starting material fails to meet the requirements for further processing, our customer care team will contact you to discuss how to proceed with your project. Whenever possible, Eurofins Genomics will recommend additional measures for optimising sample quality.
NGSelect Amplicon Adaptor Ligation:
Eurofins Genomics performs the 2nd PCR step with optimised PCR conditions and highest success rates. Still the success depends on various factors, including the amount of PCR product with universal adaptors. In case the 2nd PCR does not result in an amplicon and if we rate another trial as promising based on our experience, Eurofins Genomics will repeat the amplicon generation one-time. In case the repetition is not successful, Eurofins Genomics will stop the processing of the relevant samples and reimburse you for the sequencing part that was not performed. In the event that only a weak amplicon can be generated, Eurofins Genomics will include those amplicons in the amplicon pool for sequencing, too. Experience shows that those amplicons will result in very few reads only and interpretation of those reads should be done with special care.
For the product NGSelect Amplicon on MiSeq, can we send samples that consist of several amplicons generated with different primer pairs?
Usually you would send per sample 1 amplicon generated with 1 primer pair. In some cases you might require however less reads per amplicon as given in the Project Specification. In such cases the individual amplicon samples you send may also consist of several amplicons. Please note, that in this case however we will not perform the clipping of primer sequences and deliver raw data only.
What are TruSeq adapter sequences?
The adapter sequences are:
Forward: 5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
Reverse: 5’-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’
I have mixed amplicons in a single sample that I want to sequence. Can I get the unique sequences from this mixture, and how can your service help with this?
Yes, you can send in a single sample containing mixed amplicons for sequencing. Our Unique Sequence Analysis service is specifically designed to handle such samples. By using advanced sequencing and bioinformatics techniques, we can accurately separate and identify each unique sequence, ensuring you get comprehensive and reliable results from your mixed amplicon sample.