Frequently Asked Questions About Our Products And Services

There are pros and cons of every sequencing method. Sanger sequencing and Oxford Nanopore sequencing (ONT) are both methods for determining the sequence of nucleotides in a piece of DNA. Typically Sanger is consider the most accurate method for short-read sequencing and NGS is better for long-read sequencing.

Overall, the choice between Sanger sequencing and ONT will depend on the specific needs of the application. Both methods have their strengths and limitations, and the appropriate method will depend on factors such as the length and quality of the DNA sample, the desired level of accuracy, and the cost and availability of the necessary equipment.

It really depends on the sample quality. We cannot guarantee the level of coverage at this time. A good sample submitted properly will typically yields hundreds or even thousands of sequencing reads. Consensus coverage depends on how many reads are full-length plasmids and how many, if any, degraded.

A coverage exceeding approximately 20x indicates a highly accurate consensus sequence.

Any number of samples is welcome. There are no minimums or limitations.

You can send it and we can sequence it, but we cannot predict or promise the analysis outcome. The customer would take on the risk of these orders.

Great news! We are actively working on bringing you a prepaid solution, which will be available very soon!

Unfortunately not. Currently, we can only accept plasmids. However, we do offer a special service for amplicons and large DNA inserts, which is available for your use. Learn more >>

Unfortunately not. This barcode is required for our internal lab processes and is essential in ensuring that we can process your samples as efficiently as possible. Thank you for your cooperation!

Yes of course.

If you don't know if there is a dropbox close by, use our dropbox location page to find out. Please click here >>

Yes, FASTQ files are delivered for every project.

There are several reasons why your sample might have failed:

1. The starting material did not consist of circular DNA.
   In our library preparation protocol, circular DNA is required as the starting material. If linear DNA is provided, it will result in sample fragmentation and subsequently lead to poor or no results.
   
   
2. Insufficient DNA concentration in prepared samples.
   The primary reason for this issue is the use of a Nanodrop device to measure DNA concentration. We strongly recommend employing a Qubit or a similar method instead.
   
   
   
3. Presence of mixed plasmid species and/or fragmented genomic DNA or fragmented plasmids within the samples.
   You may detect signs of this failure mode through a wide range of read lengths reported in the raw read length histogram.

To interpret your results you can also have a look at our troubleshooting guide. Click here >>

Please send your samples in nuclease-free water or elution buffer.

Please do not use a buffer containing EDTA.

For each run, we conduct multiple quality control (QC) measurements to assess the success of the library preparation and sequencing. If all the measurements indicate satisfactory results, we will not reprocess samples that have failed.

We will sequence your plasmids typically within 1 business day.

For plasmids > 25 kb please except a turnaround time of up to 5 business days.