INVIEW Microbiome 16S-Full-Length

Custom PCR Design

Custom PCR & Sequencing

Genotyping by Sequencing (GBS) is a high-throughput technique that leverages next-generation sequencing technologies to identify genetic variants across the genome, providing comprehensive genetic analysis. It offers a cost-effective and efficient solution for large-scale genotyping, particularly in species with complex genomes

If you prepare your own amplicons, we recommend utilizing our short- and long-read amplicon sequencing services.

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Applications

If your target is not covered by existing primer sets, we offer a custom target implementation service tailored to your needs.

Our service accommodates everything from single targets to large PCR panels with up to 1000 targets, making it ideal for genotyping by sequencing (GBS) applications.

Service

We provide optional bioinformatics such as: Variant Analysis – Germline, Variant Analysis – Somatic, CRiSPR Check Analysis, Unique Sequence Analysis, Consensus Sequence Analysis

Highlights

Highly efficient primer design and assay development
Ultra-low cost per data point, enabled by multiplexing up to 300 PCRs in a single reaction
Up to 1000 target amplicons per sample
Ideal for genotyping by sequencing applications
Services conducted in ISO 17025 certified laboratories
Comprehensive bioinformatics services
Data transfer via secure FTP

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Product Specifications & Ordering

Pre-sequencing options

Please refer to our DNA isolation guide.

We do not accept samples with higher biosafety level than S2. GMOs are only accepted with S1 level. HotShot Lysate DNA extraction services available for certain tissue and blood samples.

Starting material

Sample Type: Purified DNA
Purity (OD260/280): 1.8-2.0

Volume: 50µL
Concentration: 10-50 ng/µl
Minimum Amount: 500 ng
Resuspension Buffer Water, EB, or low TE (<0.1 mM EDTA)
Format: barcode labelled 1.5 ml safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty
Shipment Method: Room temperature/ice

Specifications

The minimum sample number per batch is 188.
Sequencing on NovaSeq with 2 x 150 paired-end read mode or MiSeq with the 2 x 300 bp paired-end read module depending on amplicon length
Highly flexible data output depending on your scientific need