Our amplicon service is designed for a population of molecules that is clonal.
What will happen if you send mixtures?
If your species are sufficiently distinct (e.g. vastly different in size or sequence), the pipeline will first attempt to make a .fasta consensus file from the highest abundance species. It will also attempt to make a consensus of other species with read counts that are >20% of that of the most abundant species. Ultimately, which species end up producing a consensus will vary depending on overall sample quality, coverage, and relative abundance/degradation of each species.
How can I sequence my amplicon mixture?
Eurofins Genomics offers a vast amplicon sequencing portfolio. You will definitely find the correct service for your needs: Amplicon portfolio>>