The right answers to frequently asked questions
Find the answers to all our products and services by clicking the links below.
What starting material can be accepted?
For a list of accepted starting material, including the relevant amounts, please refer to Table 1 in Product Specifications.
What kind of quality control do you perform?
NGSelect Amplicon on NovaSeq (150 - 270 bp):
Prior to sequencing, the quality and quantity of each sample will be determined by established methods (e.g. agarose gel analysis / Qubit® Fluorometer / NanoDrop / Agilent 2100 Bioanalyzer). Further quality controls are performed at various steps of the sequencing workflow.
NGSelect Amplicon on MiSeq (280 - 570 bp):
Beside the evaluation of the gel picture you are sending, the success of the PCR reaction serves as our quality control. After the library prep and at various steps of the sequencing workflow further quality controls are performed.
What kind of BioIT do you offer?
Affordable high quality bioinformatics analysis is available for each package (see “Product Specifications” for more.
Can I use the results for diagnostic purposes?
Yes. On request, the service can be performed under diagnostic conditions with ISO17025 certified processes.
Where do I get my results and in which form are they delivered?
The results will be delivered via your Ecom account.
Where should I send my samples?
Eurofins Genomics Europe Pharma and Diagnostics Products & Services Sanger/PCR GmbH
Jakob-Stadler-Platz 7
78467 Konstanz
Germany
For the product NGSelect Amplicon on MiSeq, can we send samples that consist of several amplicons generated with different primer pairs?
Usually you would send per sample 1 amplicon generated with 1 primer pair. In some cases you might require however less reads per amplicon as given in the Project Specification. In such cases the individual amplicon samples you send may also consist of several amplicons. Please note, that in this case however we will not perform the clipping of primer sequences and deliver raw data only.
How should I send my samples?
- Please use 1.5 ml safe-lock tubes for your templates and primers
- Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
- Label your template with our free NGS barcode labels.
If you send more than 92 samples then you can also sent the samples in plates. Please use full skirted PCR plates (e.g. Eppendorf or Twin).
For more information and for sample submission for extraction please read our Sample Submission Guide.
(Picture shows the procedure with Sanger Prepaid Barcodes but work the same with free NGS Barcodes)
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1
Only use 1.5 ml
safe-lock tubes
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2
Remove barcode
stricker from film
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3
Affix barcode sticker
horizontally
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4
QR-code visible at front so
it can be read by scaner
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