Although the gold standard for TMB determination is whole-exome sequencing (WES), focused panels, like the INVIEW Oncoprofiling, have been evaluated extensively for TMB estimation because of their lower sequencing cost and higher sensitivity. Recent research shows, that panels with a size > 1.5 Mbp are sufficiently suited to determine TMB with a high concordance to WES (Buchhalter et al. 2019, Int J Cancer 144:848–858). Thus, INVIEW Oncoprofiling's size of 3 Mbp provides more precise TMB estimates than other smaller panels.