We recommend starting with total RNA (DNA-free). For detailed sample submission requirements please visit our Sample Submission Guidelines.

To successfully extract RNA for RNA sequencing please follow these guidelines:

• Use Quality Reagents: Choose high-quality reagents and kits specifically designed for RNA extraction to ensure maximum yield and purity.
• Tissue or Cell Handling:
  • If using tissue, immediately snap-freeze or store it in RNA preservation solutions (like RNAlater) to prevent RNA degradation.
  • For cell cultures, process samples promptly and keep them on ice until extraction.
• Homogenization:
  • Homogenize the samples thoroughly to ensure complete lysis of cells and release of RNA.
• Follow Protocols Carefully: Adhere strictly to the manufacturer's protocol of the RNA extraction kit. Pay close attention to:
  • Sample input amount
  • Lysis buffer volume
  • Centrifugation times and speeds
• Avoid RNases:
  • Use RNase-free tubes, pipette tips, and reagents. Wear gloves and use sterile equipment to minimize contamination.
• Check RNA Quality and Quantity:
  • Use a spectrophotometer (e.g., NanoDrop) and a bioanalyzer / Fragment Analyzer to assess RNA purity (A260/A280 ratio) and integrity (RNA Integrity Number, RIN).
• Storage:
  • Store extracted RNA at -80°C for long-term preservation. For short-term storage, RNA can be kept at -20°C.
• Prepare for Library Construction:
  • Ensure that the RNA is free of contaminants (like DNA and proteins) and within the required concentration range for downstream applications.