The total base output of long read sequencing is highly dependent on input DNA fragment length and quality.
To estimate the coverage for long read sequence the formula is used:
(Average Read length) × (Total number of reads) ÷ (Genome size).
For example, sequencing a genome of 50 Mb with 1 million reads and an average read length of 5 kb (total base output of 1 Gb) will result in an average coverage of 100x.