NGS UDI Primer Sets
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    NGS UDI Primer Sets

     

     

    Unique Dual Index Primer Pairs for Multiplexing on Illumina 


    Eurofins Genomics' NGS UDI Primer Sets are specifically designed to prevent misassignment due to index hopping.

     

    The highlights of Eurofins Genomics' NGS UDI Primer Sets at a glance

    • Utilizes 12-nucleotide long Unique Dual Indexes (UDIs)
    • Minimum edit distance of four bases or more, enabling precise and accurate demultiplexing.
    • Designed to prevent misassignment due to index hopping.
    • Compatible with all TruSeq-based NGS library types, including those using NEB adapters.
    • Offers exceptional performance even with low-complexity pools
    • Achieving a best-in-class cross-contamination rate.
    • Each batch is quality controlled by our NGS facility and released only after meeting strict QC criteria.
    • The general structure of the primers can be found in each plate assigment document below.



    NGS UDI Primer Sets are delivered in a full-skirted 96-well PCR plate with a pierceable foil seal. Each primer pair has a concentration of 10µM provided in bidest water in 10µl for single use. Enter the quantity you need and click "Add to Cart".

     


    EF UDP 0001 - 0096


    NGS UDI Primer Set 96-01

     


    96 primer pairs for 96 reactions


    Click here to download plate assignment with u7 and u5 index primer sequences.*

     

     

    Will be available soon
         
         


    EF UDP 0097 - 0192


    NGS UDI Primer Set 96-02

     


    96 primer pairs for 96 reactions


    Click here to download plate assignment with u7 and u5 index primer sequences.*

    		  

     

     

    Will be available soon
         
         


    EF UDP 0193 - 0288


    NGS UDI Primer Set 96-03

     

    96 primer pairs for 96 reactions


    Click here to download plate assignment with u7 and u5 index primer sequences.*

    		  

     

     

    Will be available soon
         
         


    EF UDP 0289 - 0384


    NGS UDI Primer Set 96-04

     

    96 primer pairs for 96 reactions


    Click here to download plate assignment with u7 and u5 index primer sequences.*

    		  

     

     

    Will be available soon


    * For information on which u5 index orientation to use (forward or reverse complement), please refer to the following Illumina page:

    https://support-docs.illumina.com/SHARE/AdapterSequences/Content/SHARE/AdapterSeq/Overview.htm

     

    Information for the use of 12 bp UDI sequences for sequencing on MiSeq systems can be found here.

     

     

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