Optimierte Primer & Sonden - denn die Performance zählt.
Primer und Sonden müssen in Ihrer Applikation funktionieren und nicht analytischen Kriterien entsprechen.
Daher entwickeln wir stetig neue optimierte und validierte Oligos für Sie und Ihre Applikation:
- qPCR Probes
Sonden für die Real-Time PCR und FRET Assays
- PCR Primers
Der optimale Primer für jede End-Punkt- und Real-Time PCR
Perfekt für die PCR Sequenzierung mittels Sanger
- Cloning Oligos
Beste Wahl für DNA- & PCR-Klonierungen, SDM und Gensynthese
- NGSgrade Oligos
Für jede NGS Applikation, validiert von unserem NGS Labor
Unsere Optimised Application Oligos wurden für die jeweiligen Anwendungen optimiert und in zahlreichen Experimenten validiert.
Die vordefinierten Spezifikationen ermöglichen Ihnen noch einfacheren Bestellprozess.
Erhalten Sie Ihre optimalen Primer und Sonden mit nur ein paar Klicks in Expressgeschwindigkeit.
Das sagen unsere Kunden über unsere applikationsoptimierten Oligos
Teilen Sie uns Ihre Erfahrungen in unserem Feedback-Formular mit.
"We used the Cloning Oligo and the HYPUR oligos to synthetically make a gene containing several point mutations (gene synthesis using degenerated primers).
After cloning into our expression vector, we sequenced 46-48 clones. In term of internal deletion (usually missing 1 bp), we see the same rate between HYPUR and the Cloning Oligo. In our hand we had around 50-52% of complete gene with those primers. In term of diversity (the primer were degenerated), we found the best diversity with the Cloning Oligos (every mutation was represented 50-70% as expected). Using the HYPUR, we have some site which have 10 mutationà almost no diversity).
Next to that Cloning/HYPUR comparison, we also did 2 extra libraries using different set of primers (both Cloning Oligos). Here the percent of gene without deletion was much higher with 78% and 61%, and the diversity was as expected.
All together, and in addition to an higher level of synthesis, I believe that your Cloning Oligos seems to be of superior quality and are most suitable for our various application."
Christophe Blanchetot, PhD, FarGEN-X BVBA, Gent, Belgium
"For our PCR with subsequent cloning of the PCR products, using commercially available cloning vectors, we ordered new Cloning Oligos with lengths of 36, 57, 63 and 67 bases. 17 out of 25 clones were picked and subsequently sequenced at Eurofins.
All 17 clones contained the correct inserts. In summary, I found that the new Cloning Oligos performed equally as well as the purified HPSF oligos I regularly order."
Dr. Anton Schmitz, LIMES-Institut, Bonn, Germany
"For evaluation of the new Cloning Oligo quality, primer pairs for the amplification of 18 target sequences from cDNA samples were designed. All pairs were obtained twice, in the new Cloning Oligo quality as well as for reference purposes in regular HPSF purified form.The 18 target sequences were successfully amplified from the templates with both primer qualities. However the 3 reactions had to be repeated for the HPSF purified oligos with a newly synthesized cDNA preparation, to obtain a product. No problems occurred with the Cloning Oligos.
All amplifications were done with Phusion™ HF Polymerase from Finnzymes. Cloning of the amplified fragments was independently done for both primer qualities fragments into two plasmid vectors using regular techniques. After plasmid preparation from obtained E. coli transformants 2 clones from each reaction were tested for correctness of their sequence.
Surprisingly all clones amplified with the Cloning Oligos contained correct inserts without mutations due to the incorporated oligonucleotides. In contrast, clones from the HPSF purified oligos revealed deletions and mutations in 11% of the sequence runs.
This surprising result underlines the capabilities of the new primers and will allow us to reduce our running sequencing cost in the future."
Christoph R., Munich, Germany
"We tested these new cloning oligos in our gene synthesis application and got very good results. For most genes tested we found a correct clone in the first round of analysis. So, as these cloning oligos are well suited for gene synthesis, they should be almost perfect in "normal" cloning experiments."
Dr. Uwe Koehler, Eurofins Medigenomix, Ebersberg, Germany